Journal:

Article Title: Molecular Characterization of Multidrug Resistance in Streptococcus mitis

doi:

Figure Lengend Snippet: Results of SmaI PFGE (A) and HinfI digestion of 250-bp gyrA PCR amplicons (B) of the 14 S. mitis isolates. Lanes 1 and 2, S. mitis strains isolated from the case patient in April and May 1996, respectively; lanes 3 through 14, 12 noncase S. mitis isolates. Lanes M, Lamba Ladder PFG marker (New England BioLabs, Beverly, Mass.) (A) and the 100-bp DNA ladder (Gibco BRL, Gaithersburg, Md.) (B).

Article Snippet: Lanes M, Lamba Ladder PFG marker (New England BioLabs, Beverly, Mass.) (A) and the 100-bp DNA ladder (Gibco BRL, Gaithersburg, Md.) (B). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Isolate MIC (μg/ml) a of: Molecular characterization by: CIP ERY CLI TET PEN CTX CRO IMP VAN PCR tet (M) d hybridization gyrA b mef c ermB c Case April 1996 64 >32 >16 >16 1 0.12 0.5 1 1 + − + + May 1996 64 >32 >16 >16 1 0.5 1 0.5 1 + − + + Noncase 1 0.5 >8 >8 ≤0.5 0.5 1 1 0.06 ≤0.5 − − + − 2 0.5 ≤0.5 ≤0.5 >4 0.06 0.06 0.06 0.06 ≤0.5 − − − + 3 2 ≤0.5 ≤0.5 >4 0.03 0.06 0.06 0.03 ≤0.5 − − − + 4 2 ≤0.5 ≤0.5 ≤0.5 0.5 0.25 0.25 0.06 ≤0.5 − − − − 5 2 >8 >8 ≤0.5 0.25 0.5 0.25 0.06 ≤0.5 − − + − 6 0.5 1 ≤0.5 ≤0.5 1 0.5 0.5 0.25 ≤0.5 − + − − 7 64 >8 >8 >4 0.03 0.06 0.06 0.06 ≤0.5 + − + + 8 0.5 2 ≤0.5 ≤0.5 0.03 0.06 0.06 0.03 ≤0.5 − + − − 9 4 >8 >8 >4 0.25 1 0.5 0.06 ≤0.5 − − + + 10 1 ≤0.5 ≤0.5 ≤0.5 0.03 ≤0.015 ≤0.015 0.03 ≤0.5 − − − − 11 0.5 >8 >8 >4 >8 >4 >4 2 ≤0.5 − − + + 12 0.5 2 ≤0.5 ≤0.5 4 2 2 1 ≤0.5 − + − − Open in a separate window a CIP, ciprofloxacin; ERY, erythromycin; CLI, clindamycin; TET, tetracycline; PEN, penicillin; CTX, cefotaxime; CRO, ceftriaxone; IMP, imipenem; VAN, vancomycin. b gyrA PCR amplicons (250 bp) were digested by Hin fI.

Techniques: Isolation, Marker

Journal:

Article Title: Characterization of Group C and G Streptococcal Strains That Cause Streptococcal Toxic Shock Syndrome

doi: 10.1128/JCM.42.1.186-192.2004

Figure Lengend Snippet: PFGE separation of SmaI-restricted fragments of the chromosomal DNA of the GCS and GGS strains.

Article Snippet: Lambda DNA PFGE markers (Amersham Pharmacia Biotech) were used as size standards.

Techniques:

Journal:

Article Title: Characterization of Isolates of Methicillin-Resistant Staphylococcus aureus from Hong Kong by Phage Typing, Pulsed-Field Gel Electrophoresis, and Fluorescent Amplified-Fragment Length Polymorphism Analysis

doi: 10.1128/JCM.41.11.4980-4985.2003

Figure Lengend Snippet: PFGE profiles of SmaI macrorestriction fragments of MRSA strains. (A) eMRSA isolates. Lanes 1, 10, and 20, λ DNA PFGE markers; lane 2, ATCC 25923; lanes 3 to 9, eMRSA-1 to -7; lanes 11 to 19, eMRSA-8 to -16. (B) Representatives of international MRSA clones and major PFGE types from the PWH. Lanes 1 and 13, λ DNA PFGE markers; lane 2, ATCC 25923; lane 3, Brazilian clone (HSJ216); lane 4, Iberian clone (HPV107): lane 5, New York/Tokyo clone (BK2464); lane 6, pediatric clone (HDE288); lanes 7 to 11, representatives of major PFGE types of MRSA from the PWH (A1, B1, C1, D, and E, respectively); lane 12, internal control strain (MRSA 3).

Article Snippet: A lambda DNA PFGE molecular size standard (Amersham Pharmacia Biotech, Little Chalfont, United Kingdom) and an ATCC control strain were included in each gel.

Techniques: Clone Assay

Journal: Foodborne Pathogens and Disease

Article Title: Antibiotic Resistance Profiling, Analysis of Virulence Aspects and Molecular Genotyping of Staphylococcus aureus Isolated in Sicily, Italy

doi: 10.1089/fpd.2017.2338

Figure Lengend Snippet: Staphylococcus aureus Strains Isolated in Sicily Analyzed by Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis

Article Snippet: The DNA was digested with 20 U Sma I (New England Biolabs) at room temperature for 4 h. Macrorestriction fragments were separated using a BioRad CHEF System (30" 12 h, 15" 6 h, 1% gel in Tris-borate-EDTA [TBE] 0.5 × , 200 V) and a PFGE size standard (CHEF DNA Size Standard, Lambda [λ] ladder; BioRad) was added.

Techniques: Isolation, Sequencing, Pulsed-Field Gel

Journal: Foodborne Pathogens and Disease

Article Title: Antibiotic Resistance Profiling, Analysis of Virulence Aspects and Molecular Genotyping of Staphylococcus aureus Isolated in Sicily, Italy

doi: 10.1089/fpd.2017.2338

Figure Lengend Snippet: Pulsotypes of Staphylococcus aureus isolates. (A) Example of the PFGE profiles after SmaI digestion of genomic DNA, detected in this study. (B) Dendrogram of the ten PFGE profiles.

Article Snippet: The DNA was digested with 20 U Sma I (New England Biolabs) at room temperature for 4 h. Macrorestriction fragments were separated using a BioRad CHEF System (30" 12 h, 15" 6 h, 1% gel in Tris-borate-EDTA [TBE] 0.5 × , 200 V) and a PFGE size standard (CHEF DNA Size Standard, Lambda [λ] ladder; BioRad) was added.

Techniques: